منابع مشابه
Microencapsulated antibodies in radioimmunoassay. III. Determination of cortisol.
We describe a method for directly measuring total plasma cortisol by use of a microencapsulated antibody. The antibody microcapsules were incubated with plasma and 125I-labeled cortisol in a competitive reaction at 37 degrees C for 15 min, then separated by centrifugation; the radioactivity of the pellet was counted for 1 min. Analytical recovery of cortisol from three plasma pools was 97.6% (S...
متن کاملTriiodothyronine radioimmunoassay.
Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3) and several triiodoanalogues were potent ...
متن کاملRadioimmunoassay for measuring antibodies specific for group B streptococcal types Ia, Ib, Ic, II, and III.
The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing human sera for group B type-specific antibodies, since it requires very small qu...
متن کاملComparison of commercial kits for radioimmunoassay: III. Radioassay of serum folate.
We evaluated six commercial radioassay kits in which tritium or iodine tracers are used to measure serum folate. We assessed the adequacy of the literature supplied with the kits, within-run precision, reagent stability, analytical recovery of added pteroylglutamic acid and N-methyltetrahydrofolic acid, results of analysis of patients' samples, and minimum detection limits. There were serious d...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1971
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.20.9_484